Western Blot:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Immunohistochemistry:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Flow Cytometry:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Immunofluorescence:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Immunohistochemical staining:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Staining:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 